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Haemolytic anaemia: investigation

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Escalate

Suspected thrombotic microangiopathy with thrombocytopenia and schistocytes, acute intravascular haemolysis with haemoglobinuria or kidney injury, a haemolytic transfusion reaction, severe infection-associated haemolysis, or anaemia with shock, chest pain, syncope or heart failure requires same-hour senior haematology and transfusion support. Draw diagnostic and blood-bank samples promptly, but do not delay organ support or a TTP pathway.

Synopsis

Confirm accelerated red-cell destruction, separate intravascular from extravascular and immune from non-immune mechanisms, and recognise fragmentation, transfusion reactions and inherited disorders requiring urgent action.

  • Confirm haemolysis with a pattern, not a solitary LDH: falling haemoglobin, raised absolute reticulocytes, unconjugated bilirubin and LDH, reduced haptoglobin and supportive film findings should be reconciled with timing and liver function.
  • A low reticulocyte response does not exclude dangerous haemolysis early in the episode or when infection, marrow disease, renal failure, iron, B12 or folate deficiency limits compensation.
  • The direct antiglobulin test detects immunoglobulin or complement on red cells; it supports immune classification but does not by itself prove that active haemolysis is occurring.

Key red flags

Intravascular haemolysis

Dark urine, haemoglobinaemia or haemoglobinuria, markedly consumed haptoglobin, renal injury and rapid symptoms indicate free haemoglobin released within the circulation.

Investigation priorities

01
FBC, absolute reticulocytes and serial haemoglobinFirst step

Quantify anaemia, compensation and tempo across all lineages.

Management branches

Unstable or fragmentedAct on organ-threatening haemolysis

There is shock, severe symptomatic anaemia, haemoglobinuria with kidney injury, thrombocytopenia with fragments, or a suspected transfusion reaction.

  1. Use ABCDE, stop any transfusion, maintain venous access, check identity and send urgent FBC, film, reticulocytes, haemolysis, renal, coagulation, group and blood-bank reaction samples while supporting circulation and urine output.
  2. The preferred emergency route is immediate senior haematology and transfusion discussion, with a TTP pathway for high-probability microangiopathy and the local transfusion-reaction pathway for temporally related haemolysis.
Confirm and classifyBuild a concordant haemolysis pattern

Anaemia, jaundice, reticulocytosis, dark urine or an abnormal film raises red-cell destruction in a stable patient.

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Sources and review status4 sources · checked 27 Aug 2026 · clinical review pending
Sources

Sources and review status

National guidance is shown before implementation-dependent detail. Typical adult dose examples remain subject to patient factors, contraindications and the live BNF or specialist protocol. Source check completed 27 Aug 2026; clinical approval remains outstanding.

Authoring stateRapid draftClinical stateAwaiting reviewJurisdictionUnited Kingdom